Morgan Q. Goulding

Morgan Q. Goulding

Americus, Georgia

Georgia Southwestern State University

Assistant Professor

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Published on Dec 20, 2020

Numerous tiny pink blobs

I wasn't expecting to report anything only 48 hours into the trial, but I've seen something maybe never seen before, and I can't keep it to myself. Egg production does seem already to be depressed ...

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Published on Dec 18, 2020

Starting new trial: Btk vs Bg (M)

Doing things differently in some ways this time. Only two groups per treatment, not four. Groups consist of ten snails each as before, but they get a much bigger volume of artificial pondwater - 15...

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Published on Dec 05, 2020

Sublethal effects of low-dose Bt: potential for population control

In a four-week trial, snails were fed on alternating days with chow ('snail gel') made with (1, 10, 100, or 1000 ppm) or without Dipel Dust.They were good snails and always ate all their chow.Bt ha...

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Published on Nov 24, 2020

Difficulties with anti-diphospho-ERK mAb

Difficulties with anti-diphospho-ERK mAbOne of the aims of this project is to visualize the short-range intercellular signaling that patterns the early embryo's secondary axis. Cell ablation experi...

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Published on Nov 17, 2020

Gene Transcription Not Necessary(??)

A little digression - thank you for your patience. I've been working on an extensive set of experiments where embryos are bathed, during certain developmental time periods, in seawater containing a...

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Published on Nov 14, 2020

These Winkles! More about them, and also zombies.

These winkles will probably eat anything that has nutrient value.Including the bodies left behind by you and me and everyone else.But they shouldn't be called zombie winkles just because they'd eat...

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Published on Nov 14, 2020

Big Thanks to Backers!

Thanks so much for your support... I will keep y'all posted with progress! Love, MQG

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Published on Nov 12, 2020

Completing the plan

...the body plan laid out in "fate-restricted" regional founder cells, thanks to intercellular signaling initiated by the 3D 'organizer' cell - the third macromere of the D quadrant, and mother cel...

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Published on Nov 12, 2020

Further in spiral cleavage

Further in spiral cleavageIf you want to understand the embryo better, here is the next segment of my lightboard chalk talk. 10 minutes on Yout Ube...

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Correction: the dose was about 0.3 micrograms per snail per day - not 30 micrograms. Darn powers of ten - if I were better at figuring them in my head I might be a millionaire instead of a thousandaire. Well, so the poison is a hundred times more potent than I thought - and might sterilize snails at a dose that doesn't actually hurt insects. Wow. Need to repeat this experiment. Extraordinary claims require extraordinary evidence.
Dec 07, 2020
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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PPS. Of course the median lethal dose of pesticides (for humans) is established based on experiments feeding the poisons to some other kind of mammal, rats mice and so on...
Dec 06, 2020
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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PS '...according to the US Code of Federal Regulations...' (This is talking about human toxicity of pesticides!) https://www.govinfo.gov/content/pkg/CFR-2005-title40-vol23/pdf/CFR-2005-title40-vol23-sec156-64.pdf
Dec 06, 2020
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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Dipel Dust is a commercial formulation of Bt kurstaki, a strain known to especially target larvae of lepidoptera (moth and butterfly). The label says it contains 0.064% Bt fermentation solids - all the rest is filler. ~~~ How much 'Bt fermentation solids' did each snail eat per day on average? 1.56 gdd/1000 mLsg * 0.064 gBt/100 gdd * 6.25 mLsg/chunk * 1 chunk/2 days * 1/10 snails = 3.1 x 10^-7 gBt/snail day ... So with the '1ppm' treatment, each snail ate, on average, about 30 micrograms Bt per day. ((( gdd = grams of dipel dust mLsg = milliliters of snail gel food gBt = grams of Bt fermentation solids ~~~~ Now if such a dose reduces reproductive output by more than 50%, then just how nasty a reproductive poison are we looking at? ... Estimating the snail's body weight (not counting the shell) at about half a gram, then that 1ppm dose is about 60 mg poison per kg body weight (mg/kg). ... The worst class of poison for humans, according to the US Code of Federal Regulations, is defined as 50% lethal at a dose below 50 mg/kg. ~~~~ How potent is the sterilizing effect on snails compared to the killing effect on insects? Actually not too much different. I am reading about a median lethal concentration in caterpillar food (LC50) of about 22 ppm.
Dec 06, 2020
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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The caterpillars all died - they needed an experienced caretaker. That's OK - they were only there as a positive control, in case the Bt had no effect on snails. It turns out the Bt works on snails!
Dec 05, 2020
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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I prefer to watch at 1.25x speed.
Nov 05, 2020
Single-cell analysis of whole-body pattern formation: Mama had a baby, why did its head pop off?
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Cool, I want to see them growing! Also, what about trophoblast organoids? Isn't this the kind of cell that the virus has to get through to infect the embryo/fetus?
Nov 01, 2020
Modeling Zika virus transmission from mother to child using uterine mini-organs
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Today (Tuesday 30 July): uneaten portions replaced with fresh helpings. (Same batch of gelled bacteria suspension, but kept at 4C for the past five days.)
Jul 30, 2019
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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Here's a silent movie: https://www.youtube.com/watch?v=njQdmhfAyRc
Jul 30, 2019
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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Here's a movie: https://www.youtube.com/watch?v=tIOkVtCXdxY
Jul 29, 2019
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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Here's a movie: https://www.youtube.com/watch?v=As7Rq1lBCR4
Jul 29, 2019
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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PS. For gel analysis of DNA and RNA, planning to try this newish method that is supposed to be a lot faster and better than what everybody always does! Here: https://www.ncbi.nlm.nih.gov/pubmed/15517972
Jul 25, 2019
Eco-friendly and affordable molecular weaponry against snails that transmit schistosomiasis
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